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human oral squamous cell carcinoma line scc4  (ATCC)


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    ATCC human oral squamous cell carcinoma line scc4
    Characterization of <t>SCC4-EGFP</t> spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).
    Human Oral Squamous Cell Carcinoma Line Scc4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+oral+squamous+cell+carcinoma+line+scc4/SCC-4/pmc10797594-135-0-10
    Average 96 stars, based on 753 article reviews
    human oral squamous cell carcinoma line scc4 - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids"

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    Journal: Bioconjugate Chemistry

    doi: 10.1021/acs.bioconjchem.3c00406

    Characterization of SCC4-EGFP spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).
    Figure Legend Snippet: Characterization of SCC4-EGFP spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).

    Techniques Used:

    Fluorescence imaging of DFAC-treated SCC4 spheroids. (A) Experimental scheme of SCC4-EGFP 3D spheroid treatment with DFAC and imaging. (B) IVIS fluorescence imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned at the top, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the left. (C) Quantification of fluorescence signals from the IVIS images is represented as the tumor spheroid-to-background ratio. Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way ANOVA with Tukey’s test for post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: Fluorescence imaging of DFAC-treated SCC4 spheroids. (A) Experimental scheme of SCC4-EGFP 3D spheroid treatment with DFAC and imaging. (B) IVIS fluorescence imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned at the top, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the left. (C) Quantification of fluorescence signals from the IVIS images is represented as the tumor spheroid-to-background ratio. Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way ANOVA with Tukey’s test for post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: Fluorescence, Imaging

    Photoacoustic imaging of DFAC-treated SCC4 spheroids. (A) Photoacoustic imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned on the left, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the top. (B) Quantification of photoacoustic signals from the images represented as the normalized photoacoustic signal intensity per mm 3 . Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way Brown–Forsythe and Welch ANOVA test with post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: Photoacoustic imaging of DFAC-treated SCC4 spheroids. (A) Photoacoustic imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned on the left, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the top. (B) Quantification of photoacoustic signals from the images represented as the normalized photoacoustic signal intensity per mm 3 . Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way Brown–Forsythe and Welch ANOVA test with post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: Imaging

    Long-term response of SCC4 spheroids to DFAC-mediated PIT. (top) Experimental schedule for the long-term response of SCC4 spheroids to PIT. Cells were seeded in CellCarrier Spheroid ULA 96-well microplates, followed by incubation with DFAC for 24 h. The spheroids were then irradiated with varying light doses of 10–100 J/cm 2 , followed by imaging for 5 weeks. (bottom left) Maximum intensity projection (MIP) of fluorescence signals from SCC4 spheroids on day 31 post-PDT. Scale bar corresponds to 500 μm. (bottom right) Relative viability of SCC4 spheroids treated with PIT is shown in the right panel.
    Figure Legend Snippet: Long-term response of SCC4 spheroids to DFAC-mediated PIT. (top) Experimental schedule for the long-term response of SCC4 spheroids to PIT. Cells were seeded in CellCarrier Spheroid ULA 96-well microplates, followed by incubation with DFAC for 24 h. The spheroids were then irradiated with varying light doses of 10–100 J/cm 2 , followed by imaging for 5 weeks. (bottom left) Maximum intensity projection (MIP) of fluorescence signals from SCC4 spheroids on day 31 post-PDT. Scale bar corresponds to 500 μm. (bottom right) Relative viability of SCC4 spheroids treated with PIT is shown in the right panel.

    Techniques Used: Incubation, Irradiation, Imaging, Fluorescence

    Recurrence of SCC4 spheroid growth after DFAC-mediated PIT. SCC4 spheroids with different volumes were treated with PIT with different light doses: (A) 5 J/cm 2 , (B) 25 J/cm 2 , (C) 50 J/cm 2 , and (D) 100 J/cm 2 .
    Figure Legend Snippet: Recurrence of SCC4 spheroid growth after DFAC-mediated PIT. SCC4 spheroids with different volumes were treated with PIT with different light doses: (A) 5 J/cm 2 , (B) 25 J/cm 2 , (C) 50 J/cm 2 , and (D) 100 J/cm 2 .

    Techniques Used:

    Related Articles

    Fluorescence:

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids.
    Article Snippet: Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.. Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: A Dual Function Antibody Conjugate Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624 TM , Manassas, VA, USA).. All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).

    Imaging:

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids.
    Article Snippet: Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.. Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: A Dual Function Antibody Conjugate Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624 TM , Manassas, VA, USA).. All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).

    Incubation:

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids.
    Article Snippet: Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.. Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: A Dual Function Antibody Conjugate Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624 TM , Manassas, VA, USA).. All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).

    Irradiation:

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids.
    Article Snippet: Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.. Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: A Dual Function Antibody Conjugate Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624 TM , Manassas, VA, USA).. All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).

    Control:

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30–001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids.
    Article Snippet: Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.Oral Squamous Cell Carcinoma Line (SCC4) Culture and Generation of EGFP-Expressing SCC4 Cells.. Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).. All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).All cells were cultured in DMEM:F12 medium (SH30271.01, Cytiva, Marlborough, MA) supplemented with an antibiotic mixture containing penicillin (100 IU/mL) and streptomycin (100 μg/mL) (30−001-Cl, Corning, Corning, NY), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone, Marlborough, MA), and 400 ng/ mL hydrocortisone (MilliporeSigma, Burlington, MA).

    Article Title: A Dual Function Antibody Conjugate Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids
    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624 TM , Manassas, VA, USA).. All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).All cells were cultured in DMEM:F12 Medium (SH30271.01, Cytiva, Marlborough, MA, USA) supplemented with an antibiotic mixture containing Penicillin (100 I.U/mL) and streptomycin (100 μg/mL) (30-001-Cl, Corning, Corning, NY, USA), 10% heat-inactivated fetal bovine serum (FBS) (SH30071.03HI, Hyclone TM , Marlborough, MA, USA) and 400 ng/mL hydrocortisone (MilliporeSigma, Burlington, MA, USA).



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    human oral squamous cell carcinoma line scc4  (ATCC)
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    ATCC human oral squamous cell carcinoma line scc4
    Characterization of <t>SCC4-EGFP</t> spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).
    Human Oral Squamous Cell Carcinoma Line Scc4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+oral+squamous+cell+carcinoma+line+scc4/SCC-4/pmc10797594-135-0-10
    Average 96 stars, based on 1 article reviews
    human oral squamous cell carcinoma line scc4 - by Bioz Stars, 2026-10
    96/100 stars
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    Image Search Results


    Characterization of SCC4-EGFP spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Characterization of SCC4-EGFP spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques:

    Fluorescence imaging of DFAC-treated SCC4 spheroids. (A) Experimental scheme of SCC4-EGFP 3D spheroid treatment with DFAC and imaging. (B) IVIS fluorescence imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned at the top, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the left. (C) Quantification of fluorescence signals from the IVIS images is represented as the tumor spheroid-to-background ratio. Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way ANOVA with Tukey’s test for post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Fluorescence imaging of DFAC-treated SCC4 spheroids. (A) Experimental scheme of SCC4-EGFP 3D spheroid treatment with DFAC and imaging. (B) IVIS fluorescence imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned at the top, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the left. (C) Quantification of fluorescence signals from the IVIS images is represented as the tumor spheroid-to-background ratio. Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way ANOVA with Tukey’s test for post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: Fluorescence, Imaging

    Photoacoustic imaging of DFAC-treated SCC4 spheroids. (A) Photoacoustic imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned on the left, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the top. (B) Quantification of photoacoustic signals from the images represented as the normalized photoacoustic signal intensity per mm 3 . Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way Brown–Forsythe and Welch ANOVA test with post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Photoacoustic imaging of DFAC-treated SCC4 spheroids. (A) Photoacoustic imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned on the left, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the top. (B) Quantification of photoacoustic signals from the images represented as the normalized photoacoustic signal intensity per mm 3 . Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way Brown–Forsythe and Welch ANOVA test with post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: Imaging

    Long-term response of SCC4 spheroids to DFAC-mediated PIT. (top) Experimental schedule for the long-term response of SCC4 spheroids to PIT. Cells were seeded in CellCarrier Spheroid ULA 96-well microplates, followed by incubation with DFAC for 24 h. The spheroids were then irradiated with varying light doses of 10–100 J/cm 2 , followed by imaging for 5 weeks. (bottom left) Maximum intensity projection (MIP) of fluorescence signals from SCC4 spheroids on day 31 post-PDT. Scale bar corresponds to 500 μm. (bottom right) Relative viability of SCC4 spheroids treated with PIT is shown in the right panel.

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Long-term response of SCC4 spheroids to DFAC-mediated PIT. (top) Experimental schedule for the long-term response of SCC4 spheroids to PIT. Cells were seeded in CellCarrier Spheroid ULA 96-well microplates, followed by incubation with DFAC for 24 h. The spheroids were then irradiated with varying light doses of 10–100 J/cm 2 , followed by imaging for 5 weeks. (bottom left) Maximum intensity projection (MIP) of fluorescence signals from SCC4 spheroids on day 31 post-PDT. Scale bar corresponds to 500 μm. (bottom right) Relative viability of SCC4 spheroids treated with PIT is shown in the right panel.

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: Incubation, Irradiation, Imaging, Fluorescence

    Recurrence of SCC4 spheroid growth after DFAC-mediated PIT. SCC4 spheroids with different volumes were treated with PIT with different light doses: (A) 5 J/cm 2 , (B) 25 J/cm 2 , (C) 50 J/cm 2 , and (D) 100 J/cm 2 .

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Recurrence of SCC4 spheroid growth after DFAC-mediated PIT. SCC4 spheroids with different volumes were treated with PIT with different light doses: (A) 5 J/cm 2 , (B) 25 J/cm 2 , (C) 50 J/cm 2 , and (D) 100 J/cm 2 .

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: